Keeffe debuted for the Lions in 2019 and played 11 games over three years. She had a breakout season in 2020 where she played seven games and was a part of the Club’s finals campaign.
However, she struggled in 2021 and was unable to add to her tally.
The 24-year-old has cited ongoing challenges with her mental health as the reason behind her decision to retire.
“This has easily been the hardest decision I have ever had to make and has not been made lightly,’’ she said.
“Unfortunately, the physical and mental demands of football that I used to embody and thrive off have become the exact things that have negatively affected my general well-being and therefore a lot of aspects of my life outside of football.
“I would like to say that the support from the Club and all the girls has made me feel incredibly lucky to be a part of something so special.’’
Lions Head of Womens Football Bree Brock said Keeffe would be sorely missed but applauded her courage in deciding to focus on her wellbeing.
“Jessy is a much-loved figure within our Club and she’ll leave a huge hole,’’ she said.
“She will always remain a part of the Brisbane Lions family and we will continue to support her in any way we can.
“We wish her all the best and can’t wait to see her at Lions games in the future.’’